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Studies have revealed that NNMT is distinctly overexpressed in these established cancer models

It may be because of the short treatment course in our study (50 2 days) or continuing of FM medications such as duloxetine or tricyclic antidepressants in the study time which could attenuate the vitamin B12 effect

Cells were resuspended in 35 mL binding buffer (1X PBS, 1 mM EDTA, 1 mM DTT, Protease inhibitor) then 250 units of benzonase nuclease (Sigma) were added to the resuspension

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